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ANALYSIS

Use AFM to see our DNA origami

Aim

・See the confirmation of structure assembly by AFM

Materials

Buffer: 1*TAE, 20mM-Mg2+
DNA information:
Anealing:50h
concentration:1nM
Purified in a tabletop centrifuge. Filter: AMICON 100Kf
[Purification protocol]
4500 x g: 5 min.
1000 x g: 2 min.
Repeat three times for all annealing time samples

Results

Fig.5 is the image we observe by AFM.We can't observe any DNA Origami.

soup top photo
Figure.5 Observe DNA origami by AFM